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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… facing humanity. A major focus are biofuel feedstock species and plant-microbe interactions, advancing efforts to … the globe to identify genes to improve our domesticated species.  The program supports the development of … manipulation in the laboratory. Both germplasm (sequenced mutants and natural accessions) and protocols …

… and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, … volume 20, 721–736 JC Villada, F Schulz (2022) The endosymbiotic box of protective tricks. Nature Reviews …

… 1st. Three days of great science, and all online and free. We had to cancel last year’s event due to COVID, but … holding the meeting virtually, and registration is totally free. It’s a great opportunity to learn more about JGI, hear … to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We …

… bit of background on this: Most of the machinery in any living organism are proteins, which are made up of chains of … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… that you find in plants, fungi and bacteria, basically all living things. When your crazy Aunt is posting on Facebook … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … at MIT, and I studied microbial ecology. I studied Vibrio species in the ocean, so heterotrophic bacteria. Now, we …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … Fleming – the discovery of penicillin from the fungal species – probably told us that fungi could be very …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … as being the standard set of shared reactions that most living organisms share – have together. Obviously that is … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … a microcolony, microbial aggregate, or a specific subset of free-living cells. Extracted DNA may be amplified using …

… we’re doing here. If you have suggestions for others feel free to let me know by emailing [email protected], or … able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … we’re thinking they’re obligate marine. There’s so far no species or no strains known that really grow in freshwater. … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …