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… a couple biology classes in undergrad, but my PhD was an actinobacteria. And I tell all my students this now, but … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… recent work like ActDES, which is a curated database of actinobacteria for evolutionary studies, and hopefully we … least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

So we do a couple of different things. Most of them are actinobacteria. And so we get a lot of them from databases … The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to … take one of those and co-culture them with say one of our Actinobacteria what will happen? Will it create chemistry …

November 11, 2022, in Cell Genomics , the team focused on Actinobacteria ( Actinomycetota ), a group of bacteria with … Group. Many of these bacterial and archaeal genome sequences have been generated using techniques that the JGI … Over the years more than 3,400 genomes have been sequenced from curated DSMZ isolates, for which quality DNA …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … a laboratory. Initially, my group really focused on marine actinobacteria. Then we moved into marine proteobacteria. …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … that are living there. And we’ve moved recently from actinobacteria now. And we’re focusing much of our efforts …

… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …