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The Sequencing Technologies Group applies cutting edge molecular capabilities and state-of-the-art next-generation sequencing to enable biological discovery by the JGI and external DOE users. Adopting the latest  sequencing technologies has allowed the JGI to increase …

… of the process needed to prepare and ship samples for sequencing by the JGI.  Additionally it includes … material is one of the greatest predictors of a successful sequencing project. It is imperative that users properly … nucleic acid quantification in our Illumina and PacBio sequencing workflows and provide guidance as to how to …

… any work can begin, including the submission of samples for sequencing, or the submission of sequencing data for synthesis. This agreement, required by … for Federal Agencies , DNA Synthesis Appendix  and DNA Sequencing Appendix are available for download.)  After your …

FY 2025 DOE Metrics Sequencing Quarter Total Bases (Trillions) Operating Hours …     Q4 2025 175     2,167     *Includes Illumina and PacBio sequencing platforms. **Operating Hours target is based on … even though an individual may have several projects/roles. Sequencing Quarter Total Bases (Trillions) Operating Hours …

The JGI has diverse capabilities in: DNA and RNA sequencing, Sample and library preparation, DNA synthesis … of standard analysis.  Raw data is provided for all sequencing products below. In addition, JGI will submit raw … have been  discontinued , including smRNA, bisulfite sequencing, and ChIP-seq. … Scientific Program Product …

… environmental sustainability, and bioproducts. By sequencing and analyzing the genomes of these organisms, the … … The Fungal & Algal Program harnesses cutting-edge sequencing technologies and advanced genomic analysis to … and biogeochemistry. The program’s focus is on large-scale sequencing efforts that survey the vast phylogenetic and …

PI and receive permission for their planned work.   … For sequencing or metabolomics projects: data are subject to a  … Policy … Data Management at the JGI … Policy Definitions … Sequencing and Metabolomics projects … DNA synthesis …

The JGI’s Microbial Program focuses on using cutting-edge sequencing, annotation and analysis techniques to explore … … The Microbial Program focuses on using advanced sequencing technologies to explore microbial diversity — … datasets, particularly for uncultivated microorganisms, by sequencing large populations of bacteria and archaea in …

The Metagenome Program uses advanced sequencing technologies to decode the genomes of microbes in … … The Metagenome Program focuses on the high-throughput sequencing of microbial communities to uncover the complex … of phylogenomic novelty: Advances in metagenomic sequencing, assembly and binning have enabled reconstruction …

… resources empower students through isolation and genome sequencing of new fungal species.  Students collect fungi, … into iNaturalist. Myco-Ed has:  ~50 lineages selected for sequencing 16 genomes released 20 members from 15 … laboratories with minimal overhead (cost of media, ITS sequencing and tissue preparation), allowing participation …

… plan is written into a Statement of Work document. … For sequencing projects, once work is under way, raw sequence … in publication of results. … Submission … More on Sequencing Projects …