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… to regulate Earth's carbon balance, to exploring the genetic basis of how plants have naturally adapted to varied … It also utilizes advanced tools, including deep learning algorithms for gene annotation and protein folding models, to improve the accuracy of functional predictions and speed up genome analysis. Additionally, the …

… and BiG-SLiCE, and now the BiG-FAM database of biosynthetic gene cluster families. And he tells us about forthcoming … Island. And was working on a natural products/biosynthetic gene cluster database, and a tool for predicting natural … of collaboration as well– was the integration of the RODEO algorithm by the group of Doug Mitchell from Illinois . So …

… the active site of an enzyme. So there is no promiscuity, generally speaking, in primary metabolism. Nevertheless, … BARONA-GOMEZ: OK so the EvoMining idea starts– it’s another algorithm that we have developed in the lab inspired by … such that you can really put a confidence value into your prediction. Otherwise it would have been like a very broad …

… metabolomics fuels the discovery  of novel metabolites, genes, and proteins. It also enables exploration of energy … JGI scientists are able to link metabolic activity with gene expression, providing deeper insights into functional … a web-based service returns a list of scored reaction and gene matches associated with metabolites and a list of …

… conditions, with only a small portion of the biosynthetic gene clusters (BGCs) being expressed. The program works to … uncover these hidden biochemical resources by exploring the genetic pathways responsible for their synthesis. The … their binding sites across multiple genomes. Computational prediction of heterologous expressability: We are developing …

… could have arisen from. DAN UDWARY: OK, so sort of a phylogenetic reconstruction kind of a thing. ALISON NARAYAN: … work with in the lab. That’s something– ancestral protein prediction, reconstruction is a whole field. There’s a lot … necessarily know if I buy into that. I’m curious about the algorithms that are used to do these predictions. And if …

… and he has a project with the JGI to synthesize the genes for and explore the chemistry of quorum sensing … And Alison has done a really nice job of providing that general scientific audience whereas now I’m sitting here … And I was really fascinated by that. And really more generally from undergrad the intersection of chemistry and …

… of it. So it wasn’t only chemistry or only biochemistry or genetics. It was also ecology. It was also evolution. It was … I could see a certain time span within these biosynthetic gene clusters. There’s gene fusion going on, and then I … see if you can see patterns and if you can actually make predictions. And then you get excited if you can make …

… oftentimes will bind to receptors within biosynthetic gene clusters. Specifically, these are Tet repressors that oftentimes turn off these biosynthetic gene clusters. And so we've been really interested in trying … for NRPSs nearby, and then we used both AntiSMASH and PRISM predictions to get an idea of what that NRPS peptide might …

After I got my undergraduate degree, which was in general biology, I joined the Peace Corps. And when I was … looking at that I’m a very visual person. So, when we make genetic mutants in a fungal isolate, I can see something … are encoding the same things. And so you have to have good algorithms. And one interesting thing is though, you might …

America brought to the UK? ROGER:  Yeah, it’s difficult to generalize, of course, but I felt overall that the British … and curate the information and make sure that what the algorithm had done was actually accurate. And so we told … is still quite tricky. You can’t easily do it by forward prediction from the gene cluster. Not to the discrete …

… bit more into fungi and learning the differences of all the genetic differences and then expanded on on that knowledge … mentoring. I enjoyed teaching and just helping the next generation of researchers in natural products and in other … genomes. We did comparative analysis. We did structure prediction. And it was amazing. And now you have– just to …