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… about microbiology and natural products from the Great Salt Lake, and you’re going to learn, like me, what a “haboob” … bit more into fungi and learning the differences of all the genetic differences and then expanded on on that knowledge … of Utah , about her secondary metabolism research on novel microbes and bioactive compounds in the Great Salt Lake. …

… metabolomics fuels the discovery  of novel metabolites, genes, and proteins. It also enables exploration of energy … processes, development of high performance plants and microbes, and understanding microbiome interactions. The  … to link metabolic activity with gene expression, providing deeper insights into functional genomics. A key focus is …

At the end of the day, all the content produced by microbes the result of millions of years of evolution. And … the active site of an enzyme. So there is no promiscuity, generally speaking, in primary metabolism. Nevertheless, … antibiotic is really an antibiotic in nature, what is the general belief is that that is the case when there is a …

I was imagining some massive contraption for amplifying genes. I wasn’t really sure what actinobacteria were. Dan … what’s important in terms of the chemistry produced by microbes. And by what’s important, I mean, like, where … to you, but they’re unprecedented in the terms biosynthetic gene clusters and genome size. And they’re so complex. …

… oftentimes will bind to receptors within biosynthetic gene clusters. Specifically, these are Tet repressors that oftentimes turn off these biosynthetic gene clusters. And so we've been really interested in trying … fun that we've started that is a different subject is from microbes to medicine labs. So this is-- we've been working …

… and he has a project with the JGI to synthesize the genes for and explore the chemistry of quorum sensing … put into antiSMASH after downloading it was isolated from Lake Washington. So it’s really interesting that it was … ecology community to be able to better predict how their microbes are interacting with each other from sequence …

… to regulate Earth's carbon balance, to exploring the genetic basis of how plants have naturally adapted to varied … climate and soil conditions around the globe to identify genes to improve our domesticated species.  The program … evolution. It also utilizes advanced tools, including deep learning algorithms for gene annotation and protein …

It was unpublished at that point. But people would push out gene clusters maybe once a month at most. Maybe twice a … have to do a 10,000-fold dilution to plate that out. The deeper you go in the cave, the less dilution. You can be … them on a regular basis. So they are full of terrestrial microbes. So they’re open ecosystems. They’re not sealed off …

… not. And so the question of do you need something that’s promiscuous for it to be useful? Not in a process chemistry … could have arisen from. DAN UDWARY: OK, so sort of a phylogenetic reconstruction kind of a thing. ALISON NARAYAN: … got a more thermally stable protein, less working in the cold room. You know? DAN UDWARY: That’s right. Yeah. JACKIE …

… and BiG-SLiCE, and now the BiG-FAM database of biosynthetic gene cluster families. And he tells us about forthcoming … all based on antiSMASH results. And so if you want to do deeper analysis on things, then you’re staying in the “BiG” … a nice example of going from a whole community with many microbes, hundreds of gene clusters, and then being able to …

… activity. So the chemistry and function really of those microbes that live there.  And in our work we’ve determined … gone to retrieve some bacteria that like it to be really cold. Can you tell us more about that.  MARCY: Sure. In … products is that we don’t know what all the biosynthetic gene clusters look like.  DAN: For sure.  MARCY: We have so …

… was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods was these damn … that can’t live without their host. They have no extra genes hanging around, they still dedicate 20-30% of their … there’s hot spots of really potent antibiotics. There’s cold spots where the pathogens are taking over and …