Robust analysis of 5 ‘-transcript ends (5 ‘-RATE): a novel technique for transcriptome analysis and genome annotation
Complicated cloning procedures and the high cost of sequencing have inhibited the wide application of serial analysis of gene expression and massively parallel signature sequencing for genome-wide transcriptome profiling of complex genomes. Here we describe a new method called robust analysis of 5′-transcript ends (5′-RATE) for rapid and cost-effective isolation of long 5′ transcript ends… [Read More]