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… there. This is Dan again, and you’re about to listen to the 3rd and final part of Natural Prodcast’s “Primer” episodes. … want to go back and listen to the first two parts ( part 1 is here and part 2 is here ) so you understand what’s … I hope you’ll stick around. But, for now, here’s episode 3 of the Primer! — DAN: So, you know, in the in the 50s 60s …

… welcome back to Natural Prodcast, and this will be episode 11. This continues our little sprint on genome mining. In … that I was exchanging with people. So I think I wrote 3,000 emails in the course of one and a half year while … some people went as far as saying, OK, we’re going to take 300 strains from our labs and measure them on their mass …

… pairs. It was not linear, like Streptomyces , but circular. 10% of the genome encoded natural product pathways. Dan: … Brad: Yeah, at that time, that was new, right? Like, my god 10% of the genome makes small molecules that have, you know, … with us? Brad:  Unfortunately, not. We lost him about 13 years ago. So, I wish he was still with us because I know …

… they had the same conserved polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: … And I set that up. And we finally got that to work 10 years later. But in the meantime there, we had people … anybody in natural product discovery in any field and even 30 years ago would say it’s the [key to] success. And that …

… like a nice summer day in Seattle I think this was like 2013 or 2014. And antiSMASH — I know you’ve interviewed Marnix … as much about it as possible starting with we picked 183 of them. And we sent that list to the Joint Genome … and an unusual acyl-homoserine lactone signal . mBio. 2023 Aug 31;14(4):e0101023. doi: 10.1128/mbio.01010-23. …

… that were acquired through it were usually only around 30% complete.  Described in  ISME Communications , a method … like plasmids, the picture is far from complete.  In 2021 , scientists at St. Jude Children’s Research Hospital … at least 80% complete when studied with PTA, compared to <30% complete when studied with MDA. With the introduction of …