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… which was a very early genome mining company, back when DNA sequencing of natural product pathways was hard and … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … it was a big deal. And you really had– I mean, the whole sequencing endeavor, had a cost, how much blood, sweat, and …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … that I never anticipated starting– that’s not why I came to the University of Utah– is looking at the … to grow. If you hear Nigel talk, he is very interested in sequencing a lot more natural product-heavy culture …

… the Broad that was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods … So you were saying, Mark, how the DNA that was breaking the sequencing from really being functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … about his move to microbiology, about drug discovery and why it’s so hard to succeed, and we talk about an ongoing … bring it into the lab. And I think that criterion explains why the natural products in the ocean weren’t studied up …

… other omics technologies.  The exponential growth of DNA sequencing data, coupled with recent computational … (Nature 2019). Other projects in the group include the sequencing and comparative analysis of thousands of archaeal … et al. (2014) Genomic Encyclopedia of Bacteria and Archaea: Sequencing a Myriad of Type Strains. PLoS Biology …

… The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … technology.] BETSY PARKINSON: OK. DNA affinity purification sequencing. And so the way this essentially works is you … at the college level. So-- DAN: Yeah, for sure. And JGI is sequencing quite a few of the things that end up-- BETSY …

… your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … JGI and this was before they were integrated. So I thought, why not feed it in there. And I went to group meeting and … AARON PURI: Yeah. No, you’re exactly right. So you can see why these are tightly regulated. DAN: For sure. For sure. …

… and the 90s. And so we started to be able to explore DNA sequencing and actually get to the DNA sequence – sequences … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … We have a lot more data. Obviously, there’s been a lot of sequencing going on, especially at the JGI and in other …

… before– or kind of around about when the first genome sequencing was done. But natural products– marine natural … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … around in natural products for a lot longer than genome sequencing, when we do a mass spec– a mass spec is like a …

… Sorghum genome serves as a reference for the highly complex … genomic breeding in these crops. For diploid species, sequencing programs generally focus on a genotype with two … hampered by the crop’s highly polyploid genome, which makes sequencing and assembly of the genome extremely challenging. …

… of critical minerals and materials. For accepted proposals, sequencing, synthesis, metabolomics and analysis will be …