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… The JGI has diverse capabilities in: DNA and RNA sequencing, Sample and library preparation, DNA synthesis … of standard analysis.  Raw data is provided for all sequencing products below. In addition, JGI will submit raw … have been  discontinued , including smRNA, bisulfite sequencing, and ChIP-seq. …

… other omics technologies.  The exponential growth of DNA sequencing data, coupled with recent computational … (Nature 2019). Other projects in the group include the sequencing and comparative analysis of thousands of archaeal and bacterial type strains ( GEBA-type strains project ), the …

… colleagues. Aaron is doing some cool stuff with cool bacteria that I’m fascinated to learn more about. He works … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … JGI and this was before they were integrated. So I thought, why not feed it in there. And I went to group meeting and …

… on synthetic biology of engineering gene expression in bacteria – however, the professor was actually quite … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … gene clusters. And I had heard the first rumors of people sequencing like these enormous numbers of genomes, like 20 …

… the Broad that was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … So, one of those is Acinetibacter . This is a genus of bacteria that’s really popped up at funding agencies and the …

… Bill Fenical. And we connected to JGI through the Community Sequencing Program, and they worked with us to sequence two … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … Initially, my group really focused on marine actinobacteria. Then we moved into marine proteobacteria. Not a …

… For sequencing or metabolomics projects: data are subject to a  … Sequencing and Metabolomics projects …

… in Nature and Medicine.” It’s obviously focused more on the bacterial side of the field, and it’s a little hard to find … and the 90s. And so we started to be able to explore DNA sequencing and actually get to the DNA sequence – sequences … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … on identifying and characterizing haloperoxidases from bacteria. And with Christian, I started getting a little bit … to grow. If you hear Nigel talk, he is very interested in sequencing a lot more natural product-heavy culture …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … [project] with JGI, how many flavin monooxygenases are you sequencing, sort of along that vein, of if you have one or … guess with along that line, with JGI, like how many are you sequencing currently? ALISON NARAYAN: Yeah. So– yeah, with …

… things, because a lot of what we do at JGI in terms of the sequencing that we do is very people-disconnected, and … a check-up on me. And I wonder how that intersects with bacterial bioprospecting that is often done, where we’re … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? …

… which was a very early genome mining company, back when DNA sequencing of natural product pathways was hard and … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … in the marine ecosystems and marine actinomycetes and cyanobacteria. And we thought we could also– let’s see what caves …