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… really the same kind of thing. Before the age of genome sequencing, we had to basically break up the pieces of DNA … And you would scan those pieces by hybridization or maybe sequencing if you were rich enough. [CHUCKLES] And once you … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … technology.] BETSY PARKINSON: OK. DNA affinity purification sequencing. And so the way this essentially works is you … OK. Oh, awesome. DAN: So yeah. If you get any cool strains you need some help, we'll probably be able to help …

… The Metagenome Program uses advanced sequencing technologies to decode the genomes of microbes in … The Metagenome Program focuses on the high-throughput sequencing of microbial communities to uncover the complex … Exploration of phylogenomic novelty: Advances in metagenomic sequencing, assembly and binning have enabled reconstruction …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … to grow. If you hear Nigel talk, he is very interested in sequencing a lot more natural product-heavy culture … and you might have the same cluster and all these different strains. But only one produces the compound. So I think …

… – problems that might need special technology for DNA sequencing, genomics, metabolomics, and synthetic biology, … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … the JGI. And so now here at the JGI, I work with all of the sequencing data that the JGI produces to try to find and …

… For sequencing projects, once work is under way, raw sequence … More on Sequencing Projects …

… already had a fermentation process with already engineered strains, but this was to further build on those to try and … NIGEL MOUNCEY: Oh, sure. I think our legacy obviously is in sequencing.  DAN: Of course.  NIGEL MOUNCEY: Sequencing the wazoo out of anything that’s relevant to the …

… before– or kind of around about when the first genome sequencing was done. But natural products– marine natural … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … And we’ve given them genome sequences of some of these strains. They’re not all Pseudonocardia . There’s other very …

… Science program. It used to actually stand for Community Sequencing Program. Also CSP, same acronym. But as JGI, as a … facility, evolved, and as we started to do more than just sequencing, we thought the name community sequencing program … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …

… you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … took an approach that was kind of a simplified metagenome sequencing approach to pick this system apart.  DAN:  And … tells you what some of those design rules might be and constrains hypotheses that you can test about how to put things …

… For accepted proposals, sequencing, synthesis, metabolomics and analysis will be … with DOE mission-relevant projects that call for expansive sequencing, synthetic biology, and metabolomics capabilities … Letters of Intent. Proposals may include synthetic biology, sequencing, and metabolomics. Proposals may include …