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… and the 90s. And so we started to be able to explore DNA sequencing and actually get to the DNA sequence – sequences … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … We have a lot more data. Obviously, there’s been a lot of sequencing going on, especially at the JGI and in other …

… The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … technology.] BETSY PARKINSON: OK. DNA affinity purification sequencing. And so the way this essentially works is you … at the college level. So-- DAN: Yeah, for sure. And JGI is sequencing quite a few of the things that end up-- BETSY …

… your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … JGI and this was before they were integrated. So I thought, why not feed it in there. And I went to group meeting and … AARON PURI: Yeah. No, you’re exactly right. So you can see why these are tightly regulated. DAN: For sure. For sure. …

… Apple Podcasts or wherever you’re getting this and tell me why you’re listening. DAN: But, now, here’s Natural Prodcast … by the National Institutes of Health. DAN:  All right, why don’t you tell us a little bit about that. ROGER:  Yeah, … us, we want to hear from you! We have projects in genome sequencing, DNA synthesis, transcriptomics, metabolomics, …

… For accepted proposals, sequencing, synthesis, metabolomics and analysis will be … with DOE mission-relevant projects that call for expansive sequencing, synthetic biology, and metabolomics capabilities … Letters of Intent. Proposals may include synthetic biology, sequencing, and metabolomics. Proposals may include …

… it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … everything is funding, right, isn’t it? I thank JGI for sequencing a lot of fungi. But the thing with them – to get … sequence is really cheap. I’ve got colleagues that are sequencing dozens and dozens of fungi, mostly coming out of …

… before– or kind of around about when the first genome sequencing was done. But natural products– marine natural … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … around in natural products for a lot longer than genome sequencing, when we do a mass spec– a mass spec is like a …

… resources empower students through isolation and genome sequencing of new fungal species.  Students collect fungi, … into iNaturalist. Myco-Ed has:  ~50 lineages selected for sequencing 16 genomes released 20 members from 15 … laboratories with minimal overhead (cost of media, ITS sequencing and tissue preparation), allowing participation …

… community at large with access to high-throughput sequencing, synthesis, and metabolomics at the JGI for … based on their DOE relevance.  For accepted proposals, sequencing, synthesis, metabolomics and analysis will be …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … really exciting work on figuring out how to get genomic sequencing data for the fungus garden because it’s more … in this project, the interaction with JGI was really around sequencing for, I think, for the fungus garden and some of …

… biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … gene clusters. And I had heard the first rumors of people sequencing like these enormous numbers of genomes, like 20 … families across all genomes. And the acceleration of genome sequencing, especially in bacteria, has been enormous. There …

… Overview The JGI provides sequencing, synthesis, and targeted metabolomics support for … a core facility instead. Proposals may not exceed 10 Tbp sequencing, 500 kbp synthesis, and 200 polar/500 nonpolar … to expand on their DOE-BER funded work with additional sequencing, synthesis, or metabolomics should apply to one …