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… DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … is similar to primary metabolism. So if you were, you know, sequencing an organism for the first time, and you’ve never … more knowledge you can generate. So that’s why we are still sequencing lots of stuff, and why the JGI is a key driver in …

… Science program. It used to actually stand for Community Sequencing Program. Also CSP, same acronym. But as JGI, as a … facility, evolved, and as we started to do more than just sequencing, we thought the name community sequencing program … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …

… it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … everything is funding, right, isn’t it? I thank JGI for sequencing a lot of fungi. But the thing with them – to get … sequence is really cheap. I’ve got colleagues that are sequencing dozens and dozens of fungi, mostly coming out of …

… The JGI’s Microbial Program focuses on using cutting-edge sequencing, annotation and analysis techniques to explore … The Microbial Program focuses on using advanced sequencing technologies to explore microbial diversity — … datasets, particularly for uncultivated microorganisms, by sequencing large populations of bacteria and archaea in …

… you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … took an approach that was kind of a simplified metagenome sequencing approach to pick this system apart.  DAN:  And … doing was pursuing with Jacques Ravel, at TIGR, metagenome sequencing, which was very early days for that technology. …

… which was a very early genome mining company, back when DNA sequencing of natural product pathways was hard and … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … it was a big deal. And you really had– I mean, the whole sequencing endeavor, had a cost, how much blood, sweat, and …

… and the 90s. And so we started to be able to explore DNA sequencing and actually get to the DNA sequence – sequences … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … We have a lot more data. Obviously, there’s been a lot of sequencing going on, especially at the JGI and in other …

… For accepted proposals, sequencing, synthesis, metabolomics and analysis will be … with DOE mission-relevant projects that call for expansive sequencing, synthetic biology, and metabolomics capabilities … Letters of Intent. Proposals may include synthetic biology, sequencing, and metabolomics. Proposals may include …

… things, because a lot of what we do at JGI in terms of the sequencing that we do is very people-disconnected, and … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? … to understand more about plant natural products? We have sequencing facilities, DNA synthesis, metabolomics. Where do …

… the Broad that was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods … So you were saying, Mark, how the DNA that was breaking the sequencing from really being functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways …

… Bill Fenical. And we connected to JGI through the Community Sequencing Program, and they worked with us to sequence two … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … at Monterey Bay, to be able to then do, at real time, sequencing on the underwater rover while it’s in the middle …

… The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … technology.] BETSY PARKINSON: OK. DNA affinity purification sequencing. And so the way this essentially works is you … at the college level. So-- DAN: Yeah, for sure. And JGI is sequencing quite a few of the things that end up-- BETSY …