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… – problems that might need special technology for DNA sequencing, genomics, metabolomics, and synthetic biology, … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … the JGI. And so now here at the JGI, I work with all of the sequencing data that the JGI produces to try to find and …

… as exploring and exploiting biocatalysts – that is, using enzymes to do chemical reactions. Obviously, in my work, in … enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … [project] with JGI, how many flavin monooxygenases are you sequencing, sort of along that vein, of if you have one or …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … about his move to microbiology, about drug discovery and why it’s so hard to succeed, and we talk about an ongoing … 20S proteasome. The proteasome in the cell is a bundle of enzymes that is used to degrade proteins, peptides, that are …

… really the same kind of thing. Before the age of genome sequencing, we had to basically break up the pieces of DNA … out the last few pieces and trying to understand the enzymology. So we would actually express the genes and do the … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… things, because a lot of what we do at JGI in terms of the sequencing that we do is very people-disconnected, and … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? … to understand more about plant natural products? We have sequencing facilities, DNA synthesis, metabolomics. Where do …

… For sequencing or metabolomics projects: data are subject to a  … Sequencing and Metabolomics projects …

… started collaborating with Ben and his group to work on sequencing, synthesis and genome mining technology to help … the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … thought. I’m going to combine natural products isolation, enzymology, and writeable recombinant DNA technology to study …

… “Non-Ribosomal Peptide Synthetases” or NRPSs. These are enzyme systems that grab amino acids and stick them together … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … took an approach that was kind of a simplified metagenome sequencing approach to pick this system apart.  DAN:  And …

… it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … everything is funding, right, isn’t it? I thank JGI for sequencing a lot of fungi. But the thing with them – to get … backbone gene synthases, but having a different decoration enzyme actually could give you something that’s quite …

… Science program. It used to actually stand for Community Sequencing Program. Also CSP, same acronym. But as JGI, as a … facility, evolved, and as we started to do more than just sequencing, we thought the name community sequencing program … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …

… But on the other hand, we're also really interested in enzymes to do some of the reactions that chemists find really … The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … technology.] BETSY PARKINSON: OK. DNA affinity purification sequencing. And so the way this essentially works is you …

… The Metagenome Program uses advanced sequencing technologies to decode the genomes of microbes in … The Metagenome Program focuses on the high-throughput sequencing of microbial communities to uncover the complex … Exploration of phylogenomic novelty: Advances in metagenomic sequencing, assembly and binning have enabled reconstruction …