AddAdapters is designed for grading the performance of adapter-trimming tools. It can add adapters to reads, and annotate the reads with their correct post-trimming length; and it can be run on trimmed reads, to calculate the rates of correct and incorrect trimming. However, it does not understand insert size, so for adding adapters to paired reads, it’s better to use RandomReads.
To add adapters to reads:
addadapters.sh in=a.fq out=b.fq adapters=adapters.fa
To grade trimmed reads:
addadapters.sh in=trimmed.fq grade
To use RandomReads instead, to add adapters in the correct location according to insert size:
randomreads.sh ref=ref.fa out=reads.fq len=150 paired reads=100k mininsert=50 maxinsert=350 fragadapter1=ACTG fragadapter2=ACTG
rename.sh in=reads.fq out=renamed.fq renamebytrim interleaved
The result of this will still be named correctly for grading by addadapters. “ACTG” would normally be a much longer adapter sequence.