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    Data yielded from RIViT-seq increased the number of sigma factor-gene pairs confirmed in Streptomyces coelicolor from 209 to 399. Here, grey arrows denote previously known regulation and red arrows are regulation identified by RIViT-seq; orange nodes mark sigma factors while gray nodes mark other genes. (Otani, H., Mouncey, N.J. Nat Commun 13, 3502 (2022). https://doi.org/10.1038/s41467-022-31191-w)
    Streamlining Regulon Identification in Bacteria
    Regulons are a group of genes that can be turned on or off by the same regulatory protein. RIViT-seq technology could speed up associating transcription factors with their target genes.

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    (PXFuel)
    Designer DNA: JGI Helps Users Blaze New Biosynthetic Pathways
    In a special issue of the journal Synthetic Biology, JGI scientific users share how they’ve worked with the JGI DNA Synthesis Science Program and what they’ve discovered through their collaborations.

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    A genetic element that generates targeted mutations, called diversity-generating retroelements (DGRs), are found in viruses, as well as bacteria and archaea. Most DGRs found in viruses appear to be in their tail fibers. These tail fibers – signified in the cartoon by the blue virus’ downward pointing ‘arms’— allow the virus to attach to one cell type (red), but not the other (purple). DGRs mutate these ‘arms,’ giving the virus opportunities to switch to different prey, like the purple cell. (Courtesy of Blair Paul)
    A Natural Mechanism Can Turbocharge Viral Evolution
    A team has discovered that diversity generating retroelements (DGRs) are not only widespread, but also surprisingly active. In viruses, DGRs appear to generate diversity quickly, allowing these viruses to target new microbial prey.

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    Photograph of a stream of diatoms beneath Arctic sea ice.
    Polar Phytoplankton Need Zinc to Cope with the Cold
    As part of a long-term collaboration with the JGI Algal Program, researchers studying function and activity of phytoplankton genes in polar waters have found that these algae rely on dissolved zinc to photosynthesize.

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    This data image shows the monthly average sea surface temperature for May 2015. Between 2013 and 2016, a large mass of unusually warm ocean water--nicknamed the blob--dominated the North Pacific, indicated here by red, pink, and yellow colors signifying temperatures as much as three degrees Celsius (five degrees Fahrenheit) higher than average. Data are from the NASA Multi-scale Ultra-high Resolution Sea Surface Temperature (MUR SST) Analysis product. (Courtesy NASA Physical Oceanography Distributed Active Archive Center)
    When “The Blob” Made It Hotter Under the Water
    Researchers tracked the impact of a large-scale heatwave event in the ocean known as “The Blob” as part of an approved proposal through the Community Science Program.

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    A plantation of poplar trees. (David Gilbert)
    Genome Insider podcast: THE Bioenergy Tree
    The US Department of Energy’s favorite tree is poplar. In this episode, hear from ORNL scientists who have uncovered remarkable genetic secrets that bring us closer to making poplar an economical and sustainable source of energy and materials.

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    HPCwire Editor's Choice Award (logo crop) for Best Use of HPC in the Life Sciences
    JGI Part of Berkeley Lab Team Awarded Best Use of HPC in Life Sciences
    The HPCwire Editors Choice Award for Best Use of HPC in Life Sciences went to the Berkeley Lab team comprised of JGI and ExaBiome Project team, supported by the DOE Exascale Computing Project for MetaHipMer, an end-to-end genome assembler that supports “an unprecedented assembly of environmental microbiomes.”

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    With a common set of "baseline metadata," JGI users can more easily access public data sets. (Steve Wilson)
    A User-Centered Approach to Accessing JGI Data
    Reflecting a structural shift in data access, the JGI Data Portal offers a way for users to more easily access public data sets through a common set of metadata.

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    Phytozome portal collage
    A More Intuitive Phytozome Interface
    Phytozome v13 now hosts upwards of 250 plant genomes and provides users with the genome browsers, gene pages, search, BLAST and BioMart data warehouse interfaces they have come to rely on, with a more intuitive interface.

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    screencap from Amundson and Wilkins subsurface microbiome video
    Digging into Microbial Ecosystems Deep Underground
    JGI users and microbiome researchers at Colorado State University have many questions about the microbial communities deep underground, including the role viral infection may play in other natural ecosystems.

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    Yeast strains engineered for the biochemical conversion of glucose to value-added products are limited in chemical output due to growth and viability constraints. Cell extracts provide an alternative format for chemical synthesis in the absence of cell growth by isolating the soluble components of lysed cells. By separating the production of enzymes (during growth) and the biochemical production process (in cell-free reactions), this framework enables biosynthesis of diverse chemical products at volumetric productivities greater than the source strains. (Blake Rasor)
    Boosting Small Molecule Production in Super “Soup”
    Researchers supported through the Emerging Technologies Opportunity Program describe a two-pronged approach that starts with engineered yeast cells but then moves out of the cell structure into a cell-free system.

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    These bright green spots are fluorescently labelled bacteria from soil collected from the surface of plant roots. For reference, the scale bar at bottom right is 10 micrometers long. (Rhona Stuart)
    A Powerful Technique to Study Microbes, Now Easier
    In JGI's Genome Insider podcast: LLNL biologist Jennifer Pett-Ridge collaborated with JGI scientists through the Emerging Technologies Opportunity Program to semi-automate experiments that measure microbial activity in soil.

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    A view of the mangroves from which the giant bacteria were sampled in Guadeloupe. (Hugo Bret)
    Giant Bacteria Found in Guadeloupe Mangroves Challenge Traditional Concepts
    Harnessing JGI and Berkeley Lab resources, researchers characterized a giant - 5,000 times bigger than most bacteria - filamentous bacterium discovered in the Caribbean mangroves.

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    In their approved proposal, Frederick Colwell of Oregon State University and colleagues are interested in the microbial communities that live on Alaska’s glacially dominated Copper River Delta. They’re looking at how the microbes in these high latitude wetlands, such as the Copper River Delta wetland pond shown here, cycle carbon. (Courtesy of Rick Colwell)
    Monitoring Inter-Organism Interactions Within Ecosystems
    Many of the proposals approved through JGI's annual Community Science Program call focus on harnessing genomics to developing sustainable resources for biofuels and bioproducts.

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    Coloring the water, the algae Phaeocystis blooms off the side of the sampling vessel, Polarstern, in the temperate region of the North Atlantic. (Katrin Schmidt)
    Climate Change Threatens Base of Polar Oceans’ Bountiful Food Webs
    As warm-adapted microbes edge polewards, they’d oust resident tiny algae. It's a trend that threatens to destabilize the delicate marine food web and change the oceans as we know them.

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August 12, 2014

Genome sequencing of 100 Thioalkalivibrio strains

The project aims to sequence the genomes of 100 strains of the genus Thioalkalivibrio. Members of this genus have an enormous metabolic and genetic diversity. Isolated from various soda lakes around the world, the strains were selected for their industrial relevance in the sustainable removal of sulfur from waste streams and energy carriers, such as… [Read More]

August 12, 2014

Microbes that thrive in serpentinized environments

Serpentinization is a common process that is extremely important for transporting water towards the mantle and for fixing large amounts of carbon as carbonate rocks. The Cedars Peridotite is a site in Northern California where active serpentinization occurs, resulting in spring waters that are so extreme that no current paradigms of microbial metabolism are compatible… [Read More]

August 12, 2014

Developing Schizophyllum commune as a model system

The study of wood breakdown into simple sugars is an important field of research due to its possible application in biofuel production. Developing S. commune as a model system to study the processes of wood degradation would allow researchers to study roles of enzymes in a fungus for which exist many protocols for studying genes… [Read More]

August 12, 2014

Arctic microbial community diversity

The poles are still one of them most unexplored ecosystems on our planet in terms of microbial genetic diversity. While marine microbes in this region are known to emit greenhouse gases such as dimethyl sulfide, not much is known yet about the molecular underpinnings of these globally important processes, nor how the microbes drive these… [Read More]

August 12, 2014

Recovering freshwater metagenomes

Lakes serve as sentinels and integrators of large-scale environmental change because they respond rapidly to climatic drivers and are tightly connected to their surrounding landscapes. Microbial communities drive the flow of carbon through these lakes by processing dissolved organic carbon, breaking down particulate organic carbon, fixing and respiring large quantities of carbon dioxide, quenching methane… [Read More]

August 12, 2014

DNA & RNA datasets from forest soil communities

Forests are vast ecosystems with critical functions in global nutrient cycling. They also represent an enormous and potentially sustainable source of biomass feedstock for the production of fuels, chemicals and materials. Samples from well-characterized Long- Term Soil Productivity Study (LTSP) sites representing six distinct ecoregions across North America will be compared to reveal effects on… [Read More]

August 12, 2014

Carbon Processing in the Sea

Marine bacterioplankton comprise the largest living surface area in the sea. They detect, transport, and assimilate bioreactive constituents from the dissolved organic carbon pool, transforming them to particulate matter or recycling them back to an inorganic form. Ruegeria pomeroyi DSS-3 was isolated in 1998 from southeastern U.S. coastal seawater and has become a model organism… [Read More]

August 12, 2014

Microbial studies of Lake Erie’s “dead zone”

The Laurentian Great Lakes are the largest group of lakes on Earth, containing a fifth of near-surface and liquid freshwater globally. Lake Erie has a human-dominated watershed and has witnessed recurrent summertime oxygen depletion for a century or more. Conditions favor the formation of an expansive summer “dead zone,” comparable in surface area to the low… [Read More]

August 12, 2014

Creating Saccharomyces functional annotation libraries

Fungi are the organisms of choice as hosts for production of ethanol and other biofuels from plant material, and can serve as powerful model systems for environmental and ecological science. One of the challenges in analyzing fungal genomes is functional annotation as even in the best-studied model filamentous fungus, some 40 percent of genes have… [Read More]

August 12, 2014

Plant pathogens with supernumerary chromosomes

The Fusarium solani (FSSC) and F. oxysporum (FOSC) species complexes are two fungal clades adapted to diverse ecological niches, including plant pathogenicity on diverse hosts and the ability to engage in unique metabolic activities. Members of the FSSC and FOSC cause some of the most destructive and intractable diseases across a diverse spectrum of hosts,… [Read More]
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