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    Hyphae and asexual sporangia of Rhizopus microsporus (photo by Stephen Mondo). Inset shows a hypha with YFP-labeled Burkholderia endobacteria in green and DAPI-stained fungal nuclei in blue; scale bar, 5 um (photo by Olga Lastovetsky)
    Of Mutualism and Lipid Metabolism in Fungi
    To learn more about how bacteria interact with fungi in a symbiotic relationship to support the biochemistries that could contribute to the development of alternate fuel sources, Cornell and DOE JGI researchers used a model bacterial-fungal system to reveal the mechanism for lipid production in oil-producing fungi.

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    This graphic depicts the geographic distribution of GOLD biosamples and organisms. Organism location of isolation is marked in pink while Biosample location of collection is denoted with blue dots. Updates to the Genomes OnLine Database (GOLD) are reported in the upcoming Database issue of Nucleic Acids Research. (Image from Supratim Mukherjee et al. Nucl. Acids Res. 2016;nar.gkw992)
    DOE JGI Database of DNA viruses and retroviruses debuts on IMG platform
    In a series of four articles published in the Database issue of the Nucleic Acids Research journal, DOE JGI researchers report on the latest updates to several publicly accessible databases and computational tools that benefit the global community of microbial researchers.

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    Collecting sampled waters from Saanich inlet into carboys for large volume filtration of microbial biomass. (Image courtesy of Steven Hallam, UBC)
    Modeling Microbial Networks in an Oxygen Minimum Zone
    University of British Columbia (UBC) researchers have developed a math model that couples omic information and biogeochemical data from the Saanich Inlet ecosystem in British Columbia, Canada, which has long been used as an analog for studying oxygen minimum zones.

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  • Our Projects
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    intermediate wheatgrass
    Featured CSP FY16 Project
    Intermediate Wheatgrass Project Intermediate Wheatgrass (IWG) is a highly productive grass species that is being developed as a new biomass and grain crop. As a perennial crop, IWG can provide essential ecosystem services to current agricultural practices such as stabilizing soil with its deep root structure, reducing nutrient runoff, and limiting weed growth.

    More about this project

    Rifle, CO SFA site by RKaltschmidt
    Featured CSP FY15 Project
    Subsurface microbiology for GEWaSC modelling: Remarkably little is known about the biology of the subsurface, especially below the soil zone. In this project, researchers seek to uncover the diversity of organisms that exist in a well studied aquifer (saturated) and overlying vadose (unsaturated) zone sediments (not to include soil).

    More about this project

    The CSP project led by Clark University's David Hibbett focuses on an in-depth genomic survey of the Lentinula genus. Lentinula is a group of white-rot, wood-decaying fungi perhaps best known as the genus of shiitake mushrooms, Lentinula edodes. (Image by dominik18s via Flickr CC BY 2.0)
    CSP Plans for Fiscal Year 2016
    The CSP 2016 projects were selected from 74 full proposals received, resulting from 98 letters of intent submitted. The total allocation for the CSP 2016 portfolio is estimated to tap nearly 40 trillion bases (terabases or Tb) of the DOE JGI’s plant, fungal and microbial genome sequencing capacity.

    The complete list of FY 2016 CSP plans

  • Data & Tools
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    MetaBAT-feat
    Better Microbial Genome Binning with MetaBAT
    An automated tool called MetaBAT that automatically groups large genomic fragments assembled from metagenome sequences to reconstruct single microbial genomes.

    Read more about MetaBAT

    Photo: Roy Kaltschmidt, LBNL
    Phytozome v11 has been released!
    Phytozome is the Plant Comparative Genomics portal of the Department of Energy's Joint Genome Institute. The v11 release of Phytozome (Jan. 2016) includes a number of updates and additional data.

    Read more about the latest Phytozome release

  • User Program Info
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    011617-570-ficus-nersc
    JGI-NERSC Microbiome Data Science Call
    The Microbiome Data Science call will enable users to perform state-of-the-art computational genomics and metagenomics research and help them translate sequence information, generated by the DOE JGI or elsewhere, into biological discovery.

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    csp-feat
    Large-scale CSP Call
    The CSP Annual Call is focused on large-scale sequence-based genomic science projects that address questions of relevance to DOE missions in sustainable biofuel production, global carbon cycling, and biogeochemistry.

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    emsl-feat
    JGI-EMSL Collaborative Science Call
    The FICUS call between DOE JGI and Environmental Molecular Science Laboratory (EMSL) represents a unique opportunity for researchers to combine the power of genomics and molecular characterization in one proposed research project.

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    From sample to structure. Top: Researchers gathering samples from Great Boiling Spring in Nevada. Left: a snapshot of aligned metagenomic sequences. Each row is a different sequence (the different colors are the different amino acid groups). Each position (or column) is compared to all other positions to detect patterns of co-evolution. Bottom: the strength of the top co-evolving residues is shown as blue dots, these are also shown as colored lines on the structure above. The goal is to make a structure that makes as many of these contacts as possible. Right: a cartoon of the protein structure predicted. The protein domain shown is from Pfam DUF3794, this domain is part of a Spore coat assembly protein SafA. (Image of Great Boiling Spring by Brian Hedlund, UNLV. Protein structure and composite image by Sergey Ovchinnikov, UW)
    Seeking Structure With Metagenome Sequences
    In collaboration with researchers, an University of Washington-led team reports that structural models have been generated for 12 percent of the protein families that had previously had no structural information available.

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    Scanning electron micrograph of two cells of Fragilariopsis cylindrus. Shown are two silica shells (Frustules) in valve view. Magnification: 15,000X; scale bar: 5 μm (Image credit: Gerhard S. Dieckmann)
    Tracking Antarctic Adaptations in Diatoms
    In Nature, a team led by University of East Anglia scientists conducted a comparative genomic analysis involving three diatoms by tapping expertise from the DOE Joint Genome Institute, who conducted all sequencing and annotation.

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    A MPI video featuring study senior author Tobias Erb discussing this project may be viewed at http://bit.ly/ErbCETCH.
    Engineering a More Efficient System for Harnessing Carbon Dioxide
    Tapping the DNA synthesis expertise of the DOE JGI, a team from the Max-Planck-Institute (MPI) for Terrestrial Microbiology in Marburg, Germany has reverse engineered a biosynthetic pathway for more effective carbon fixation.

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User Program Info
Home › User Program Info › Working with JGI

Working with JGI

The DOE JGI Project Management Office (PMO) carries out a number of activities to facilitate world-class science. DOE JGI Project Managers collaborate with users to develop project requirements, define the scope of genomic work and outline roles and responsibilities resulting in a formal Statement of Work (SOW).

All work performed at the DOE JGI is initiated via the Work Initiation Process (WIP) interface. To submit a proposal, users must first log in (Note: new users of the DOE JGI will need to register and create a login at https://signon.jgi.doe.gov).

Once users are notified that their proposal has been accepted, the PMO will assist with initiation of work through:

  • Coordinating execution of Institutional User Agreements
  • Coordinating with DOE JGI technical experts
  • Project design with Principal Investigator
  • Providing a General Orientation to DOE JGI systems to Investigators
  • Preparation of formal Statement of Work (project roadmap)

Users must prepare Materials (Samples) for Submission before they can receive an Approval to Ship Samples that will be subject to the DOE JGI’s capabilities, such as Next-Gen Sequencing and/or DNA Synthesis. Once materials are received, your Project Manager will facilitate project execution by:

  • Metadata verification and coordination with IMG, GOLD and others for data annotation and submission
  • Tracking, monitoring and expediting laboratory processing
  • Consulting with investigators or DOE JGI technical staff to resolve issues, as needed

Users are notified of sample QC results, sequencing QC results (as required) and/or DNA synthesis completion. During the sample QC process, your Project Manager will consult with you to resolve any issues that may arise. You may monitor the status of your sequencing project at any time by logging on to the Genome Portals using your  DOE JGI login.

After sequencing has completed, your Project Manager will initiate analysis with the appropriate DOE JGI analysis team. During this time, your Project Manager will:

  • Coordinate JGI data analysts and other technical experts
  • Track, monitor and report analysis status to investigators
  • Facilitate data sharing with investigators through DOE JGI Genome Portals
  • Coordinate data analysis tool tutorials, as needed

Upon completion of all work associated with your proposal, your Project Manager will formally close out your project. You will be notified of the project’s completion and receive instructions for how to access your data, share with collaborators, and coordinate publication with DOE JGI scientists. Synthetic DNA constructs are also shipped at this time. Your Project Manager will, as needed:

  • Provide additional requested information or resources to users
  • Coordinate meetings with DOE JGI Science Program leads
  • Solicit user feedback about your overall experience working with the DOE JGI.

Find out more about our Project Management Office (PMO)

Project Management FAQ

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