DOE Joint Genome Institute

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    TEM image of O. tauri strain RCC4221. Credit: Herve Moreau
    Tiny Green Algae Reveal Large Genomic Variation
    A decade after the complete representative genomes of three Ostreococcus picoplankton groups were sequenced, researchers have sequenced and analyzed the genomes of 13 members of a natural Ostreococcus population.

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    Genome-wide distribution of fast neutron-induced mutations in the Kitaake rice mutant population. (Guotian Li and Rashmi Jain)
    Mutant Rice Database for Bioenergy Research
    Boosting yields of bioenergy feedstock crops such as grasses requires a functional genomics resource for grass models involved in plant cell wall biosynthesis studies. Using fast-neutron irradiation, rice researchers were able to create the first major, large-scale collection of mutations for grass models.

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    The oil slick in the Gulf of Mexico from the Deepwater Horizon oil spill as seen on April 29, 2010. (NASA Earth Observatory image created by Jesse Allen, using data provided courtesy of the University of Wisconsin’s Space Science and Engineering Center MODIS Direct Broadcast system)
    Lessons from Simulating A Deep Ocean Oil Spill
    Researchers present the first complete picture of how successive waves of microbial populations degraded the released oil. They were also able to recover high-quality genomes of the key microbial players, and determine the metabolic factors driving the shifts between microbial communities.

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  • Our Projects
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    Developing an Ice Plant Gene Atlas
    The ice plant was the first reported halophytic, facultative crassulacean acid metabolism (CAM) species, meaning that the plant can be induced to switch from C3 photosynthesis to CAM following water-deficit or salinity stress treatment.

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    Mark Dopson of Sweden’s Linnaeus University has a project that deploys DOE JGI’s single cell genomics resources on samples sourced from the deep subsurface, including the Pyhäsalmi mine. (Courtesy of Mark Dopson)
    Exploring Deep Biosphere Microbial Communities
    The data from one of the least understood habitats on Earth will have implications for our understanding of global energy and nutrient cycles, the potential for deep terrestrial disposal of nuclear waste and geo-engineering for CO2 storage, while also providing insights about how life could be sustained on other planets.

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    Suillus ampliporus, shown here, is part of the genus-wide molecular phylogeny of Suillus project from Nhu Nguyen of the University of California, Berkeley. Suillus fungi tolerate heavy metals, but the protection varies among hosts. (Image by Nhu Nguyen)
    A Suillus Genus Genome Atlas
    As symbiotic ectomycorrhizal fungi (EMF), Suillus spp. play critical roles in the function of forest ecosystems by providing their plant hosts with mineral resources in exchange for photosynthetically fixed carbon.

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  • Data & Tools
    • IMG
    • Genome Portal
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    • GOLD
    This graphic depicts the geographic distribution of GOLD biosamples and organisms. Organism location of isolation is marked in pink while Biosample location of collection is denoted with blue dots. Updates to the Genomes OnLine Database (GOLD) are reported in the upcoming Database issue of Nucleic Acids Research. (Image from Supratim Mukherjee et al. Nucl. Acids Res. 2016;nar.gkw992)
    DOE JGI Database of DNA viruses and retroviruses debuts on IMG platform
    In a series of four articles published in the Database issue of the Nucleic Acids Research journal, DOE JGI researchers report on the latest updates to several publicly accessible databases and computational tools that benefit the global community of microbial researchers.

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    Sakinaw Lake UBC
    Benchmark Study for Improving Microbial Community Profiling
    Researchers used synthetic and natural microbial lake communities to compare the microbial community profiles generated from high throughput short-read sequencing and high throughput long-read sequencing approaches.

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    Better Microbial Genome Binning with MetaBAT
    An automated tool called MetaBAT that automatically groups large genomic fragments assembled from metagenome sequences to reconstruct single microbial genomes.

    Read more about MetaBAT

  • User Program Info
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    JGI-NERSC Microbiome Data Science Call
    The Microbiome Data Science call will enable users to perform state-of-the-art computational genomics and metagenomics research and help them translate sequence information, generated by the DOE JGI or elsewhere, into biological discovery.

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    Large-scale CSP Call
    The CSP Annual Call is focused on large-scale sequence-based genomic science projects that address questions of relevance to DOE missions in sustainable biofuel production, global carbon cycling, and biogeochemistry.

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    JGI-EMSL Collaborative Science Call
    The FICUS call between DOE JGI and Environmental Molecular Science Laboratory (EMSL) represents a unique opportunity for researchers to combine the power of genomics and molecular characterization in one proposed research project.

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  • News & Publications
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    • Photos
    The release of 1,003 phylogenetically diverse bacterial and archaeal reference genomes, the single largest release to date, is part of the DOE JGI’s Genomic Encyclopedia of Bacteria and Archaea (GEBA) initiative. (Zosia Rostomian, Berkeley Lab Creative Services.)
    Uncovered: 1000 New Microbial Genomes
    DOE JGI scientists have taken a decisive step forward in uncovering the planet’s microbial diversity, reporting the release of 1,003 phylogenetically diverse bacterial and archaeal reference genomes—the single largest release to date—in Nature Biotechnology.

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    Scanning electron micrograph (SEM) of Neocallimastix californiae, a representative of the Neocallimastigomycetes, a clade of the early-diverging fungal lineages that are not well-studied. It's one of three Neocallimastigomycetes sequenced and annotated by the DOE JGI for this study. (Chuck Smallwood, PNNL)
    Fungal Enzymes Team Up to More Efficiently Break Down Cellulose
    In Nature Microbiology, a team led by researchers at UC Santa Barbara has found for the first time that early lineages of fungi can form complexes of enzymes capable of degrading plant biomass. The work was enabled by harnessing the capabilities of two U.S. Department of Energy (DOE) Office of Science User Facilities: the DOE JGI and EMSL.

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    Linderina pennispora ZyGoLife Research Consortium Flickr CC BY-SA 2.0
    Finding A New Major Gene Expression Regulator in Fungi
    DOE JGI scientists report the prevalence of a particular DNA base modification (6mA) in the earliest branches of the fungal kingdom.

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All JGI Features
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July 7, 2017

Tiny Green Algae Reveal Large Genomic Variation

Tiny Green Algae Reveal Large Genomic Variation

First complete picture of standing genetic variation within a natural population. The Science Ostreococcus are tiny green algae that are the bases of many marine food webs. A decade after the complete representative genomes of three Ostreococcus picoplankton groups were sequenced, researchers have sequenced and analyzed the genomes of 13 members of a natural Ostreococcus…

July 5, 2017

Mutant Rice Database for Bioenergy Research

Mutant Rice Database for Bioenergy Research

Genome-wide rice studies yield first major, large-scale collection of mutations for grass models. The Science Fast-neutron irradiation, exposure to high energy neutrons, induces a wide variety of mutations by making changes in DNA. Using this approach, rice researchers were able to create the first major, large-scale collection of mutations for grass models. Resequencing the 1,504…

June 30, 2017

Lessons from Simulating A Deep Ocean Oil Spill

Lessons from Simulating A Deep Ocean Oil Spill

Metagenome pipeline helps researchers resolve near-complete microbial genomes involved in Deepwater Horizon oil spill. The Science The 2010 Deepwater Horizon oil spill released 4.1 million barrels of oil into the Gulf of Mexico and was the first major release of oil and natural gases into the deep ocean (1,500 meters). Due to the depth of…

June 12, 2017

Uncovered: 1000 New Microbial Genomes

Uncovered: 1000 New Microbial Genomes

Potential biotech applications seen with release of 1,003 reference bacterial and archaeal genomes.   The number of microbes in a handful of soil exceeds the number of stars in the Milky Way galaxy, but researchers know less about what’s on Earth because they have only recently had the tools to deeply explore what is just…

May 26, 2017

Extreme Science: Sequencing in Space

Extreme Science: Sequencing in Space

On May 11, 2017, NASA astronaut and microbiologist Kate Rubins, the first woman to sequence DNA in space, gave a talk about “Science in Extreme Environments” at Lawrence Berkeley National Laboratory (Berkeley Lab). Among the anecdotes she shared about living and working in space for nearly 4 months was one on how mundane bench tasks…

May 26, 2017

Fungal Enzymes Team Up to More Efficiently Break Down Cellulose

Fungal Enzymes Team Up to More Efficiently Break Down Cellulose

Collaborative science initiative enables resolution of fungal protein complexes. One of the biggest barriers in the commercial production of sustainable biofuels is to cost-effectively break down the bioenergy crops into sugars that can then be converted into fuel. To reduce this barrier, bioenergy researchers are looking to nature and the estimated 1.5 million species of…

May 8, 2017

Finding A New Major Gene Expression Regulator in Fungi

Finding A New Major Gene Expression Regulator in Fungi

Researchers report prevalent DNA base modification in the earliest fungal lineages Just four letters – A, C, T, and G – make up an organism’s genetic code. Changing a single letter, or base, can lead to changes in protein structures and functions, impacting an organism’s traits. In addition, though, subtler changes can and do happen,…

May 1, 2017

May 11: Doing Science in Space

May 11: Doing Science in Space

With the topic “Doing Science in Space: At 17,500 miles per hour and 250 miles up,” NASA Astronaut Kate Rubins will be speaking at Lawrence Berkeley National Laboratory (Berkeley Lab) on Thursday, May 11, 2017. A livestream of the talk will be available for Berkeley Lab staff here. This visit is co-hosted by the Molecular Foundry and the…

April 28, 2017

A Gene that Influences Grain Yields in Grasses

A Gene that Influences Grain Yields in Grasses

Genetic screen identifies mutations that impact green foxtail panicle formation. The Science Through deep sequencing of the model grass green foxtail (Setaria viridis), researchers pinpointed a gene critical for the development of flowers that give rise to the grain. Using this information, a homologous gene in maize was identified as playing a similar role highlighting…

April 25, 2017

Brian Hedlund, University of Nevada, Las Vegas

Brian Hedlund, University of Nevada, Las Vegas

How long have you collaborated with the JGI and on which projects? I’ve been working with the JGI for about ten years now. To date, all of our work has focused on some aspect of life in terrestrial geothermal springs, mostly Great Boiling Spring in northeast Nevada. These projects have included work on both microbial…

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