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    TEM image of O. tauri strain RCC4221. Credit: Herve Moreau
    Tiny Green Algae Reveal Large Genomic Variation
    A decade after the complete representative genomes of three Ostreococcus picoplankton groups were sequenced, researchers have sequenced and analyzed the genomes of 13 members of a natural Ostreococcus population.

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    Genome-wide distribution of fast neutron-induced mutations in the Kitaake rice mutant population. (Guotian Li and Rashmi Jain)
    Mutant Rice Database for Bioenergy Research
    Boosting yields of bioenergy feedstock crops such as grasses requires a functional genomics resource for grass models involved in plant cell wall biosynthesis studies. Using fast-neutron irradiation, rice researchers were able to create the first major, large-scale collection of mutations for grass models.

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    The oil slick in the Gulf of Mexico from the Deepwater Horizon oil spill as seen on April 29, 2010. (NASA Earth Observatory image created by Jesse Allen, using data provided courtesy of the University of Wisconsin’s Space Science and Engineering Center MODIS Direct Broadcast system)
    Lessons from Simulating A Deep Ocean Oil Spill
    Researchers present the first complete picture of how successive waves of microbial populations degraded the released oil. They were also able to recover high-quality genomes of the key microbial players, and determine the metabolic factors driving the shifts between microbial communities.

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  • Our Projects
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    Developing an Ice Plant Gene Atlas
    The ice plant was the first reported halophytic, facultative crassulacean acid metabolism (CAM) species, meaning that the plant can be induced to switch from C3 photosynthesis to CAM following water-deficit or salinity stress treatment.

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    Mark Dopson of Sweden’s Linnaeus University has a project that deploys DOE JGI’s single cell genomics resources on samples sourced from the deep subsurface, including the Pyhäsalmi mine. (Courtesy of Mark Dopson)
    Exploring Deep Biosphere Microbial Communities
    The data from one of the least understood habitats on Earth will have implications for our understanding of global energy and nutrient cycles, the potential for deep terrestrial disposal of nuclear waste and geo-engineering for CO2 storage, while also providing insights about how life could be sustained on other planets.

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    Suillus ampliporus, shown here, is part of the genus-wide molecular phylogeny of Suillus project from Nhu Nguyen of the University of California, Berkeley. Suillus fungi tolerate heavy metals, but the protection varies among hosts. (Image by Nhu Nguyen)
    A Suillus Genus Genome Atlas
    As symbiotic ectomycorrhizal fungi (EMF), Suillus spp. play critical roles in the function of forest ecosystems by providing their plant hosts with mineral resources in exchange for photosynthetically fixed carbon.

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  • Data & Tools
    • IMG
    • Genome Portal
    • MycoCosm
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    • GOLD
    This graphic depicts the geographic distribution of GOLD biosamples and organisms. Organism location of isolation is marked in pink while Biosample location of collection is denoted with blue dots. Updates to the Genomes OnLine Database (GOLD) are reported in the upcoming Database issue of Nucleic Acids Research. (Image from Supratim Mukherjee et al. Nucl. Acids Res. 2016;nar.gkw992)
    DOE JGI Database of DNA viruses and retroviruses debuts on IMG platform
    In a series of four articles published in the Database issue of the Nucleic Acids Research journal, DOE JGI researchers report on the latest updates to several publicly accessible databases and computational tools that benefit the global community of microbial researchers.

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    Sakinaw Lake UBC
    Benchmark Study for Improving Microbial Community Profiling
    Researchers used synthetic and natural microbial lake communities to compare the microbial community profiles generated from high throughput short-read sequencing and high throughput long-read sequencing approaches.

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    Better Microbial Genome Binning with MetaBAT
    An automated tool called MetaBAT that automatically groups large genomic fragments assembled from metagenome sequences to reconstruct single microbial genomes.

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  • User Program Info
    • Community Science Program (CSP)
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    JGI-NERSC Microbiome Data Science Call
    The Microbiome Data Science call will enable users to perform state-of-the-art computational genomics and metagenomics research and help them translate sequence information, generated by the DOE JGI or elsewhere, into biological discovery.

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    Large-scale CSP Call
    The CSP Annual Call is focused on large-scale sequence-based genomic science projects that address questions of relevance to DOE missions in sustainable biofuel production, global carbon cycling, and biogeochemistry.

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    JGI-EMSL Collaborative Science Call
    The FICUS call between DOE JGI and Environmental Molecular Science Laboratory (EMSL) represents a unique opportunity for researchers to combine the power of genomics and molecular characterization in one proposed research project.

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  • News & Publications
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    • Photos
    The release of 1,003 phylogenetically diverse bacterial and archaeal reference genomes, the single largest release to date, is part of the DOE JGI’s Genomic Encyclopedia of Bacteria and Archaea (GEBA) initiative. (Zosia Rostomian, Berkeley Lab Creative Services.)
    Uncovered: 1000 New Microbial Genomes
    DOE JGI scientists have taken a decisive step forward in uncovering the planet’s microbial diversity, reporting the release of 1,003 phylogenetically diverse bacterial and archaeal reference genomes—the single largest release to date—in Nature Biotechnology.

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    Scanning electron micrograph (SEM) of Neocallimastix californiae, a representative of the Neocallimastigomycetes, a clade of the early-diverging fungal lineages that are not well-studied. It's one of three Neocallimastigomycetes sequenced and annotated by the DOE JGI for this study. (Chuck Smallwood, PNNL)
    Fungal Enzymes Team Up to More Efficiently Break Down Cellulose
    In Nature Microbiology, a team led by researchers at UC Santa Barbara has found for the first time that early lineages of fungi can form complexes of enzymes capable of degrading plant biomass. The work was enabled by harnessing the capabilities of two U.S. Department of Energy (DOE) Office of Science User Facilities: the DOE JGI and EMSL.

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    Linderina pennispora ZyGoLife Research Consortium Flickr CC BY-SA 2.0
    Finding A New Major Gene Expression Regulator in Fungi
    DOE JGI scientists report the prevalence of a particular DNA base modification (6mA) in the earliest branches of the fungal kingdom.

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Our Projects
Home › CSP Plans
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February 10, 2017

Systems Analysis of Grass Secondary Cell Wall Development

The objective of this proposal is to identify the proteins, phosphopeptides, transcripts, and metabolic signatures that mediate secondary cell wall (SCW) development in grasses. Grass cell walls are one of the most abundant potential sources of biomass for biofuel production. However, the biological conversion of grass biomass to fuel is limited by deconstruction of cell… [Read More]

February 10, 2017

Global Warming Induced Salinity Shifts

The reality of global warming predicts that the survival of many organisms will be compromised due to rapid environmental changes of the ecosystems in which they live. Aquatic ecosystems are particularly vulnerable to shifts in climate. Large incursions of fresh water or water loss from lack of rainfall will cause modification in water chemistry. As… [Read More]

February 10, 2017

Systems-Level Insights into Plant-Microbial-Permafrost Carbon Dynamics

A fundamental challenge of modern environmental science is to understand how earth systems will respond to climate change. A parallel challenge in biology is to understand how information encoded in organismal genes manifests as biogeochemical processes at ecosystem-to-global scales. These grand challenges intersect in the need to understand the global carbon (C) cycle, which is… [Read More]

February 10, 2017

Understanding Conversion of Biomass-Derived Carbon in R. toruloides

The objective of this proposal is to understand how biomass-derived carbon is converted into lipids and terpenoids in the oleaginous and carotenogenic yeast Rhodosporidium toruloides. We will carry out a detailed systems biology-level study of wild type and mutant strains using a combination of multi-omic technologies and high resolution microscopy methods. Our goal is to… [Read More]

February 10, 2017

Role of Priming Effects on Blue Carbon Conversion in the Coastal Zone

“Blue carbon,” or organic carbon (OC) derived from and stored in coastal and marine environments, is recognized as an important OC sink within the global carbon cycle, particularly in mangrove, salt marsh, and seagrass settings. However, as sea level continues to rise, these blue carbon reserves will become increasingly sensitive to wetland erosion/loss, particularly in… [Read More]

February 10, 2017

Mechanisms Underlying Changes in Forest Soils Responses

A more complete understanding of how global warming will affect carbon-cycle feedbacks to the climate system is central to model projections of future climate. Simulation experiments with fully coupled, three-dimensional carbon-climate models suggest that carbon-cycle feedbacks could either substantially accelerate or slow climate change over the 21st century. The potential switch of the terrestrial biosphere… [Read More]

February 10, 2017

Metabolic Constraints in Sediment Deposits

Depositional environments such as floodplains are poorly understood and dynamic components of the global carbon cycle that not are well represented in Earth system models. Further, they have a dominant influence on metal cycling within critical transport conduits to surface and groundwater. Importantly, the fate of carbon and redox active metals such as iron and… [Read More]

February 10, 2017

Combined ‘Omics to Study Ectomycorrhizal Symbiosis between Suillus and Pinaceae

This study will explore the genetics of obligately symbiotic interactions between conifers (Pinaceae) and their host-specific ectomycorrhizal fungi in the mushroom genus Suillus, and their effects on ecological cycling of forest carbon. Using conifer seedlings experimentally inoculated with different Suillus species, we will employ integrated metatranscriptomic / metaproteomic/metabolomic approaches identify key fungal in EMF-Pinaceae roots… [Read More]

February 10, 2017

Deciphering Controls on Plant Decomposition in Arctic Ecosystems

Climate change in the Arctic is progressing rapidly, and much of the focus, thus far, has been on permafrost thaw and the rapid mineralization of soil organic carbon (C) by soil microorganisms. However, climate change is also increasing the abundance of woody deciduous shrubs in the Arctic, altering the vegetative biochemical landscape, which could profoundly… [Read More]

February 10, 2017

Tracking Coniferous Tree Root-Associated Microbial Activity

Coniferous forests are widely distributed in temperate and boreal biomes and the fact that they efficiently store large stocks of carbon makes them globally important. Decomposer and mycorrhizal fungi are key players in the carbon and nitrogen cycles in this environment. Despite the recognition of potential effects of N availability on C cycle-related processes, detailed… [Read More]
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